Fig. 6

Differential protein expression in the hormonal clusters of testicular cancers in the TCGA cohort. Expression of 194 cancer biology-relevant proteins was investigated in cancer samples of the TCGA cohort with reverse phase protein array. \({\text{log}}_{2}\)-transformed expression levels were compared between the hormonal clusters by one-way ANOVA with \({\upeta }^{2}\) effect size statistic. Differences in \({\text{log}}_{2}\)-transformed expression between the cluster and the cohort mean were assessed by one-sample T test. P values were corrected for multiple testing with the false discovery rate (FDR) method. Proteins with pFDR(ANOVA) < 0.05, \({\upeta }^{2}\ge 0.14\), and pFDR(T test) < 0.05 were deemed differentially regulated. A Differences in \({\text{log}}_{2}\) expression between the cluster mean and the cohort mean along with FDR-adjusted p values of the T test are presented in Volcano plots. Each point represents a single gene. Point color codes for significance and regulation sign. Top most strongly regulated genes are labeled with their symbols. Numbers of significantly up- and downregulated proteins are displayed in the plot captions: (B) \({\text{log}}_{2}\)-transformed expression levels of selected proteins discriminating between the hormonal clusters. Median \({\text{log}}_{2}\) expression values with interquartile ranges are shown as boxes with whiskers spanning over 150% of the interquartile ranges. Cancer samples are visualized as points. Effect sizes and p values for comparison of the clusters with one-way ANOVA are displayed in the plot caption. Numbers of samples in the clusters are indicated in the X axes