Fig. 5

Modulation of transcriptional regulons and signaling pathways in the hormonal clusters of testicular cancer. Differential modulation of the collecTRI model transcriptional regulons and of the PROGENy signaling pathways in the hormonal clusters as comped with the cohort mean was predicted by decoupleR linear modeling tools fed with T statistics of differential gene expression for all available genes. Linear modelling score (LM score) served as an effect size metric of regulon/signaling pathway modulation (LM score < 0: inhibition, LM score > 0: activation as compared with the cohort mean). P values (LM score \(\ne\) 0) were adjusted for multiple testing with the false discovery rate method. A LM scores for top strongest modulated transcriptional regulons in the hormonal clusters (horizontal facets) found to be significant in both the TCGA and GSE99420 cohorts presented in bar plots. B LM scores for signaling pathways in the hormonal clusters found to be significantly modulated both in the TCGA and GSE99420 cohorts depicted in bubble plots. Point sizes represent absolute values of the LM scores. Point color codes for regulation sign. Points are labelled with the corresponding LM score values